DERIVATIZATION PROCEDURES FOR REDUCING OLIGOSACCHARIDES .4. USE OF GLYCOSYLAMINES IN A REVERSIBLE DERIVATIZATION OF OLIGOSACCHARIDES WITH THE 9-FLUORENYLMETHOXYCARBONYL GROUP, AND HPLC SEPARATIONS OF THE DERIVATIVES

被引：16

作者：

KALLIN, E ^{[1
]}

LONN, H ^{[1
]}

NORBERG, T ^{[1
]}

SUND, T ^{[1
]}

LUNDQVIST, M ^{[1
]}

机构：

[1] BIOCARB AB,DEPT ISOLAT & SEPARAT,S-22370 LUND,SWEDEN

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 1991年 / 10卷 / 03期

关键词：

D O I：

10.1080/07328309108543915

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, reversible derivatization procedure for reducing oligosaccharides was developed. Reaction of the model compound lacto-N-tetraose (beta-D-Gal-(1 --> 3)-beta-D-GlcNAc-(1 --> 3)-beta-D-Gal-(1 --> 4)-D-Glc) with aqueous ammonium bicarbonate gave the corresponding beta-glycosylamine, which was reacted with fluorenylmethoxycarbonyl chloride to give an N-fluorenylmethoxycarbonyl beta-glycosylamine (FMOC derivative). The free oligosaccharide could be recovered in high yield from the FMOC derivative by brief treatment with 15% aqueous ammonia. Complex oligosaccharide mixtures from human milk were derivatized with the new procedure, and excellent separations of the derivatives on straight-phase silica HPLC columns were achieved.

引用

收藏

页码：377 / 386

页数：10

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