CLONING, PHARMACOLOGICAL CHARACTERISTICS AND EXPRESSION PATTERN OF THE RAT GABA-A RECEPTOR ALPHA-4 SUBUNIT

被引：253

作者：

WISDEN, W ^{[1
]}

HERB, A ^{[1
]}

WIELAND, H ^{[1
]}

KEINANEN, K ^{[1
]}

LUDDENS, H ^{[1
]}

SEEBURG, PH ^{[1
]}

机构：

[1] CTR MOLEC BIOL,MOLEC NEUROENDOCRINOL LAB,NEUENHEIMER FELD 282,W-6900 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1991年 / 289卷 / 02期

关键词：

GABA-A; BENZODIAZEPINE RECEPTOR; INSITU HYBRIDIZATION; THALAMUS; ALPHA-SUBUNIT HETEROGENEITY;

D O I：

10.1016/0014-5793(91)81076-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA of rat brain encoding the GABA(A) receptor alpha-4 subunit has been cloned. Recombinant receptors composed of alpha-4, beta-2 and gamma-2 subunits bind with high affinity the GABA agonist [H-3]muscimol and the benzodiazepine 'alcohol antagonist' [H-3]Ro 15-4513, but fail to bind benzodiazepine agonists. The alpha-4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABA(A) receptors. The alpha-4, mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.

引用

页码：227 / 230

页数：4

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