PHOSPHORYLATION OF A PUTATIVE MYOSIN LIGHT CHAIN IN CHARA BY CALCIUM-DEPENDENT PROTEIN-KINASE

被引：22

作者：

MCCURDY, DW ^{[1
]}

HARMON, AC ^{[1
]}

机构：

[1] UNIV FLORIDA,DEPT BOT,GAINESVILLE,FL 32611

来源：

PROTOPLASMA | 1992年 / 171卷 / 1-2期

关键词：

CA-2+-DEPENDENT PROTEIN KINASE; CHARA; CYTOPLASMIC STREAMING; MYOSIN LIGHT CHAIN; PHOSPHORYLATION;

D O I：

10.1007/BF01379284

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ca2+-dependent protein kinase (CDPK) has been proposed to mediate inhibition by Ca2+ of cytoplasmiC streaming in the green alga Chara. We have identified the in vivo substrate(s) of CDPK in Chara by using vacuolar perfusion of individual internodal cells with [gamma-P-32]ATP. Phosphorylation of several polypeptides is enhanced when perfusions are performed at 10(-4) M free Ca2+ compared to < 10(-9) M free Ca2+. The Ca2+-stimulated phosphorylation of these proteins is inhibited by the presence of a monoclonal antibody to soybean CDPK. One of these proteins is 16 to 18 kDa and is recognized by an antibody against gizzard myosin light chains. These results demonstrate that in Chara, several polypeptides are phophorylated by CDPK and one of these proteins has been tentatively identified as a myosin light chain. These observations support the hypothesis that Ca2+-regulated phosphorylation of myosin is involved in the regulation of cytoplasmic streaming.

引用

页码：85 / 88

页数：4

共 22 条

[1]

CHENEY R E, 1992, Current Opinion in Cell Biology, V4, P27, DOI 10.1016/0955-0674(92)90055-H

[2]

GROLIG F, 1988, EUR J CELL BIOL, V47, P22

[3] A CALCIUM-DEPENDENT BUT CALMODULIN-INDEPENDENT PROTEIN-KINASE FROM SOYBEAN [J].