ENHANCEMENT OF ENZYME-ACTIVITY IN AQUEOUS-ORGANIC SOLVENT MIXTURES

被引：63

作者：

BATRA, R ^{[1
]}

GUPTA, MN ^{[1
]}

机构：

[1] Indian Inst Technol, DEPT CHEM, DELHI 110016, INDIA

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 10期

关键词：

D O I：

10.1007/BF01022403

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activities of poyphenol oxidase, peroxidase, acid phosphatase and trypsin were measured in the presence of four different water miscible solvents, acetonitrile; N,N, dimethylformamide; tetrahydrofuran and dioxane. Stimulation of enzymatic activities was observed in all the cases at a specific concentration range of cosolvent added which cannot be correlated either with log P or denaturation capacity. Also, no significant structural changes were observed by fluorescence studies even at the cosolvent concentrations where activity increases were significant.

引用

页码：1059 / 1064

页数：6

共 15 条

[1]

BATRA R, 1994, BIOTECHNOL APPL BIOC, V19, P209

[2] ENZYMES IN NON-AQUEOUS SOLVENTS [J].