A NEW ASSAY FOR CELL-DEATH

被引:30
作者
BELETSKY, IP
UMANSKY, SR
机构
[1] Institute of Biological Physics, U.S.S.R. Academy of Sciences, Pushchino, Moscow Region
关键词
Cell death; Cytotoxic and cytostatic effects; Ethidium bromide fluorescence; TNF;
D O I
10.1016/0022-1759(90)90381-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new assay for the evaluation of cell viability is described. It is based on the staining of dead cells and subsequently the whole cell population with ethidium bromide (EtBr). The method makes it possible to measure cytotoxic and cytostatic effects simultaneously and cell cultures grown both in suspension as well as by adherence may be assessed. The advantages and disadvantages of this assay are discussed. © 1990.
引用
收藏
页码:201 / 205
页数:5
相关论文
共 15 条
  • [1] AFANAS'EV V N, 1988, Radiobiologiya, V28, P731
  • [2] BELETSKY IP, 1989, GEN PHYSIOL BIOPHYS, V8, P381
  • [3] COFFMAN FD, 1988, LYMPHOKINE RES, V7, P371
  • [4] THE EFFECT OF CYTOTOXICITY ON DNA HISTOGRAMS
    DEITCH, AD
    [J]. CYTOMETRY, 1987, 8 (03): : 339 - 339
  • [5] ENDOGENOUS ENDONUCLEASE-INDUCED DNA FRAGMENTATION - AN EARLY EVENT IN CELL-MEDIATED CYTOLYSIS
    DUKE, RC
    CHERVENAK, R
    COHEN, JJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (20): : 6361 - 6365
  • [6] HACKETT RJ, 1988, J IMMUNOL, V140, P2639
  • [7] JIROMSKY VV, 1978, PRIBORI TEKHN EKSP, V6, P204
  • [8] JOHNSON LK, 1979, J BIOL CHEM, V254, P7785
  • [9] CACHECTIN/TNF KILLS OR INHIBITS THE DIFFERENTIATION OF 3T3-L1 CELLS ACCORDING TO DEVELOPMENTAL STAGE
    KAWAKAMI, M
    WATANABE, N
    OGAWA, H
    KATO, A
    SANDO, H
    YAMADA, N
    MURASE, T
    TAKAKU, F
    SHIBATA, S
    ODA, T
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 138 (01) : 1 - 7
  • [10] KRONKE M, 1988, CANCER RES, V48, P5417