CHARACTERIZATION OF THE PSEUDOMONAS-AERUGINOSA TRANSPOSABLE PHAGE-D3132 LEFT-END REGULATORY REGION

被引:13
作者
AUTEXIER, C [1 ]
WRAGGLEGARE, S [1 ]
DUBOW, MS [1 ]
机构
[1] MCGILL UNIV,DEPT MICROBIOL & IMMUNOL,3775 UNIV ST,MONTREAL H3A 2B4,QUEBEC,CANADA
基金
英国医学研究理事会;
关键词
TRANSPOSABLE PHAGE; D3112 LEFT END; NUCLEOTIDE SEQUENCE; REPRESSOR PROTEIN; IHF; (PSEUDOMONAS);
D O I
10.1016/0167-4781(91)90166-J
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The nucleotide sequence (2682 bp) of the left end of the Mu-like transposable bacteriophage D3112 cts15 from Pseudomonas aeruginosa was determined. A 720 bp open reading frame (ORF) is located on the bottom strand (positions 892-173), potentially encoding a polypeptide of 240 residues (M(r) = 26329). Specific binding of Escherichia coli Integration Host Factor (IHF) to a site located 907-922 bp from the D3112 left end suggests the existence of a P. aeruginosa IHF and its role, as in Mu, in the regulation of phage development.
引用
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页码:147 / 150
页数:4
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