AMPEROMETRIC FRUCTOSE SENSOR BASED ON DIRECT BIOELECTROCATALYSIS

被引：111

作者：

IKEDA, T

MATSUSHITA, F

SENDA, M

机构：

[1] Department of Agricultural Chemistry, Faculty of Agriculture, University of Kyoto, Sakyo-ku, Kyoto

来源：

BIOSENSORS & BIOELECTRONICS | 1991年 / 6卷 / 04期

关键词：

ENZYME ELECTRODE; UNMEDIATED BIOELECTROCATALYSIS; D-FRUCTOSE; AMPEROMETRIC BIOSENSOR; L-ASCORBIC ACID;

D O I：

10.1016/0956-5663(91)85015-O

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to D-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. D-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of L-ascorbic acid is also described.

引用

页码：299 / 304

页数：6

共 13 条

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