SEQUENCE OF THE GENE CODING FOR THE NEUROTOXIN OF CLOSTRIDIUM-BOTULINUM TYPE-A ASSOCIATED WITH INFANT BOTULISM - COMPARISON WITH OTHER CLOSTRIDIAL NEUROTOXINS

被引：48

作者：

WILLEMS, A ^{[1
]}

EAST, AK ^{[1
]}

LAWSON, PA ^{[1
]}

COLLINS, MD ^{[1
]}

机构：

[1] INST FOOD RES, DEPT MICROBIOL, READING RG6 2EF, ENGLAND

来源：

RESEARCH IN MICROBIOLOGY | 1993年 / 144卷 / 07期

关键词：

NEUROTOXIN; CLOSTRIDIUM-BOTULINUM; BOTULISM; NUCLEOTIDE; SEQUENCE; PCR; INFANT;

D O I：

10.1016/0923-2508(93)90004-L

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived from cloned PCR fragments demonstrated that the toxin gene encodes a protein of 1,296 amino acid residues. Comparative alignment of the derived infant BoNT/A sequence with those of other published neurotoxins revealed highest sequence relatedness with BoNT/A of classical food-borne botulism. The sequence identity between infant and classical BoNT/A was 94.9% for the light chain (corresponding to 23 amino acid changes) and 87.1% for the heavy chain (corresponding to 109 amino acid changes).

引用

页码：547 / 556

页数：10

共 19 条

[1]

BINZ T, 1990, J BIOL CHEM, V265, P9153

[2]

CAMPBELL KD, 1993, IN PRESS J CLIN MICR

[3]

Cato E. P., 1986, BERGEYS MANUAL SYSTE, P1141

[4] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[5]

EAST AK, 1992, FEMS MICROBIOL LETT, V96, P225, DOI 10.1016/0378-1097(92)90408-G

[6] NUMERICAL-METHODS FOR INFERRING EVOLUTIONARY TREES [J].

FELSENSTEIN, J .

QUARTERLY REVIEW OF BIOLOGY, 1982, 57 (04) :379-404

[7] ISOLATION OF AN ORGANISM RESEMBLING CLOSTRIDIUM-BARATI WHICH PRODUCES TYPE-F BOTULINAL TOXIN FROM AN INFANT WITH BOTULISM [J].

HALL, JD ;

MCCROSKEY, LM ;

PINCOMB, BJ ;

HATHEWAY, CL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (04) :654-655

[8]

Hatheway C., 1992, CLOSTRIDIUM BOTULINU, V1, P3

[9]

HUTSON RA, 1993, IN PRESS CURR MICROB

[10] RECOGNITION OF FUSOBACTERIUM-NUCLEATUM SUBGROUPS FN-1, FN-2 AND FN-3 BY RIBOSOMAL-RNA GENE RESTRICTION PATTERNS [J].

LAWSON, PA ;

GHARBIA, SE ;

SHAH, HN ;

CLARK, DR .

FEMS MICROBIOLOGY LETTERS, 1989, 65 (1-2) :41-45

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