STRUCTURE OF THE O-16 POLYSACCHARIDE FROM ESCHERICHIA-COLI O-16-K1 - AN NMR INVESTIGATION

被引：19

作者：

JANN, B ^{[1
]}

SHASHKOV, AS ^{[1
]}

KOCHANOWSKI, H ^{[1
]}

JANN, K ^{[1
]}

机构：

[1] MAX PLANCK INST IMMUNBIOL,D-79108 FREIBURG,GERMANY

来源：

CARBOHYDRATE RESEARCH | 1994年 / 264卷 / 02期

关键词：

ESCHERICHIA-COLI; O-16; ANTIGEN; POLYSACCHARIDE STRUCTURE; NMR SPECTROSCOPY;

D O I：

10.1016/S0008-6215(05)80014-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polysaccharide moiety of the O16 antigen (lipopolysaccharide) consists of D-glucopyranose, D-galactofuranose, L-rhamnopyranose, and 2-acetamido-2-deoxy-D-glucopyranose in the molar ratios 1:1:1:1. It is O-acetylated with one acetyl group per repeating unit. One- and two-dimensional NMR spectroscopy of the polysaccharide before and after O-deacetylation showed that the O16 polysaccharide has the structure --> 6)-alpha-D-Glcp-(1 --> 3)-alpha-L-Rhap-(1 --> 3)-alpha-D-GlcpNAc-(1 --> 2)-beta-D-Galf-(1 --> 2 \ OAc

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页码：305 / 311

页数：7

相关论文

共 23 条

[1] PREDICTION OF ANTI AND GAUCHE VICINAL PROTON-PROTON COUPLING-CONSTANTS IN CARBOHYDRATES - A SIMPLE ADDITIVITY RULE FOR PYRANOSE RINGS [J].

ALTONA, C ;

HAASNOOT, CAG .

ORGANIC MAGNETIC RESONANCE, 1980, 13 (06) :417-429

[2] HUDSONS RULES OF ISO-ROTATION AS APPLIED TO FURANOSIDES, AND THE CONFORMATIONS OF METHYL ALDOFURANOSIDES [J].

ANGYAL, SJ .

CARBOHYDRATE RESEARCH, 1979, 77 (DEC) :37-50

[3]

DABROWSKI J, 1987, METHODS STEREOCHEMIC, V9, P349

[4] CROSS-ANTIGENICITY AND IMMUNOGENICITY BETWEEN CAPSULAR POLYSACCHARIDES OF GROUP-C NEISSERIA-MENINGITIDIS AND OF ESCHERICHIA-COLI K92 [J].

GLODE, MP ;

ROBBINS, JB ;

LIU, TY ;

GOTSCHLICH, EC ;

ORSKOV, I ;

ORSKOV, F .

JOURNAL OF INFECTIOUS DISEASES, 1977, 135 (01) :94-102

[5] STRUCTURES OF THE 01B AND 01C LIPOPOLYSACCHARIDE ANTIGENS OF ESCHERICHIA-COLI [J].

GUPTA, DS ;

SHASHKOV, AS ;

JANN, B ;

JANN, K .

JOURNAL OF BACTERIOLOGY, 1992, 174 (24) :7963-7970

[6] THE O1 ANTIGEN OF ESCHERICHIA-COLI - STRUCTURAL CHARACTERIZATION OF THE O1A1-SPECIFIC POLYSACCHARIDE [J].

JANN, B ;

SHASHKOV, AS ;

GUPTA, DS ;

PANASENKO, SM ;

JANN, K .

CARBOHYDRATE POLYMERS, 1992, 18 (01) :51-57

[7] STRUCTURE OF THE O83-SPECIFIC POLYSACCHARIDE OF ESCHERICHIA-COLI O83-K24-H31 [J].

JANN, B ;

SHASHKOV, AS ;

HAHNE, M ;

KOCHANOWSKI, H ;

JANN, K .

CARBOHYDRATE RESEARCH, 1994, 261 (02) :215-222

[8] THE O-18-ANTIGENS (LIPOPOLYSACCHARIDES) OF ESCHERICHIA-COLI - STRUCTURAL CHARACTERIZATION OF THE O18A-SPECIFIC, O18A1-SPECIFIC, O18B-SPECIFIC AND O18B1-SPECIFIC POLYSACCHARIDES [J].

JANN, B ;

SHASHKOV, AS ;

GUPTA, DS ;

JANN, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 210 (01) :241-248

[9] STRUCTURAL STUDIES OF THE O-ANTIGEN POLYSACCHARIDE OF ESCHERICHIA-COLI-O4 [J].

JANSSON, PE ;

LINDBERG, B ;

OGUNLESI, M ;

SVENSON, SB ;

WRANGSELL, G .

CARBOHYDRATE RESEARCH, 1984, 134 (02) :283-291

[10] COMPUTER-ASSISTED STRUCTURAL-ANALYSIS OF POLYSACCHARIDES WITH AN EXTENDED VERSION OF CASPER USING H-1-NMR AND C-13-NMR DATA [J].

JANSSON, PE ;

KENNE, L ;

WIDMALM, G .

CARBOHYDRATE RESEARCH, 1989, 188 :169-191