3-DIMENSIONAL RECONSTRUCTION OF COXIELLA-BURNETTI-INFECTED L929-CELLS BY HIGH-VOLTAGE ELECTRON-MICROSCOPY

被引：12

作者：

HECHEMY, KE ^{[1
]}

MCKEE, M ^{[1
]}

MARKO, M ^{[1
]}

SAMSONOFF, WA ^{[1
]}

ROMAN, M ^{[1
]}

BACA, O ^{[1
]}

机构：

[1] UNIV NEW MEXICO,DEPT BIOL,ALBUQUERQUE,NM 87131

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 10期

关键词：

D O I：

10.1128/IAI.61.10.4485-4488.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previous examination of thin sections of L929 cells heavily infected with the Q fever Priscilla isolate by conventional transmission electron microscopy indicated that the rickettsiae resided within multiple vacuoles. The present study using high-voltage electron microscopy and three-dimensional reconstruction revealed that, in heavily infected cells, the rickettsiae, in fact, reside in one multilobed vacuole. As a result of asymmetric cell division, the multilobed vacuole containing the rickettsiae apparently segregates into one daughter cell, while the companion daughter cell emerges parasite free. This likely explains the appearance of naive uninfected cells in long-term-infected (i.e., ca. 2 years) cell populations that had not been supplemented with uninfected L929 host cells.

引用

页码：4485 / 4488

页数：4

共 7 条

[1] Q-FEVER AND COXIELLA-BURNETII - A MODEL FOR HOST-PARASITE INTERACTIONS [J].

BACA, OG ;

PARETSKY, D .

MICROBIOLOGICAL REVIEWS, 1983, 47 (02) :127-149

[2]

HHECHEMY KE, 1992, J MED MICROIBOL, V36, P229

[3]

KHAVKIN T, 1990, Q FEVER DISEASE, V1, P76

[4]

MALLAVIA LP, 1989, INTRACELLULAR PARASI, P117

[5] 3-DIMENSIONAL RECONSTRUCTION OF CELLS FROM SERIAL SECTIONS AND WHOLE-CELL MOUNTS USING MULTILEVEL CONTOURING OF STEREO MICROGRAPHS [J].

MARKO, M ;

LEITH, A ;

PARSONS, D .

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1988, 9 (04) :395-411

[6]

ROMAN MJ, 1991, ACTA VIROL, V35, P503

[7]

ROMAN MJ, 1986, J GEN MICROBIOL, V132, P1415

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