FORMATION OF HIGH-MOLECULAR-WEIGHT MATERIAL BY FREE-LIVING MARINE-BACTERIA

被引:40
作者
HEISSENBERGER, A [1 ]
HERNDL, GJ [1 ]
机构
[1] UNIV VIENNA, INST ZOOL, DEPT MARINE BIOL, A-1090 VIENNA, AUSTRIA
关键词
EXTRACELLULAR RELEASE; BACTERIA; TRANSFORMATION; HIGH MOLECULAR WEIGHT MATERIAL; DISSOLVED ORGANIC MATTER;
D O I
10.3354/meps111129
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Bacterial transformation of leucine into high molecular weight dissolved organic matter (HMW-DOM) material was studied in seawater cultures. Three different types of cultures were used: sodium azide poisoned cultures served as control, eukaryotic inhibitors to prevent bacterivory, and untreated cultures were used to determine the effect of grazing on bacteria on the release of HMW-DOM. All these cultures were labeled with C-14-leucine and the distribution of incorporated label into particulate organic matter (POM) and C-14 recovered in dissolved organic matter (DOM) was measured over a period of 10 d as well as the transformation into HMW-DOM. Fractionation of DOM was performed using dialysis membranes (molecular weight cutoff 50 000 daltons) and by gel filtration for substances with a molecular weight below 1500 daltons. There was a shift to HMW-DOM discernible in grazed and ungrazed cultures and this DOM fraction accumulated to ca 6% of the initially added leucine in the grazed culture within 8 d. Flagellates appeared to have only a minor influence on the formation of HMW-DOM but might be important in consuming bacterially derived HMW-DOM, representing a novel, nonpredatory way of carbon and energy transfer from bacteria to flagellates.
引用
收藏
页码:129 / 135
页数:7
相关论文
共 34 条
[1]   RELEASE OF AMINO-ACIDS AND INORGANIC NUTRIENTS BY HETEROTROPHIC MARINE MICROFLAGELLATES [J].
ANDERSSON, A ;
LEE, C ;
AZAM, F ;
HAGSTROM, A .
MARINE ECOLOGY PROGRESS SERIES, 1985, 23 (01) :99-106
[2]  
Azam F., 1987, ECOLOGY MICROBIAL CO, P261
[3]   PHYTOPLANKTON EXUDATION OF ORGANIC-MATTER - WHY DO HEALTHY CELLS DO IT [J].
BJORNSEN, PK .
LIMNOLOGY AND OCEANOGRAPHY, 1988, 33 (01) :151-154
[4]   CELL-VOLUME TO CELL CARBON CONVERSION FACTORS FOR A BACTERIVOROUS MONAS SP ENRICHED FROM SEAWATER [J].
BORSHEIM, KY ;
BRATBAK, G .
MARINE ECOLOGY PROGRESS SERIES, 1987, 36 (02) :171-175
[5]   PRODUCTION OF BIOLOGICALLY REFRACTORY DISSOLVED ORGANIC-CARBON BY NATURAL SEAWATER MICROBIAL-POPULATIONS [J].
BROPHY, JE ;
CARLSON, DJ .
DEEP-SEA RESEARCH PART A-OCEANOGRAPHIC RESEARCH PAPERS, 1989, 36 (04) :497-507
[6]   ORGANIC-CARBON RELEASE BY PHYTOPLANKTON - ITS COMPOSITION AND UTILIZATION BY BACTERIOPLANKTON [J].
CHROST, RJ ;
FAUST, MA .
JOURNAL OF PLANKTON RESEARCH, 1983, 5 (04) :477-493
[7]  
DECHO AW, 1990, OCEANOGR MAR BIOL, V28, P73
[8]  
Ducklow H. W., 1992, Environmental microbiology., P1
[9]   EXCRETION OF ORGANIC-MATTER BY PHYTOPLANKTON [J].
FOGG, GE .
LIMNOLOGY AND OCEANOGRAPHY, 1977, 22 (03) :576-577
[10]   BIOLOGICAL CONSIDERATIONS IN THE MEASUREMENT OF DISSOLVED FREE AMINO-ACIDS IN SEAWATER AND IMPLICATIONS FOR CHEMICAL AND MICROBIOLOGICAL STUDIES [J].
FUHRMAN, JA ;
BELL, TM .
MARINE ECOLOGY PROGRESS SERIES, 1985, 25 (01) :13-21