EXPRESSION OF RAT-BRAIN NITRIC-OXIDE SYNTHASE IN BACULOVIRUS-INFECTED INSECT CELLS AND CHARACTERIZATION OF THE PURIFIED ENZYME

被引：56

作者：

HARTENECK, C

KLATT, P

SCHMIDT, K

MAYER, B

机构：

[1] GRAZ UNIV, INST PHARMAKOL & TOXIKOL, A-8010 GRAZ, AUSTRIA

[2] FREE UNIV BERLIN, INST PHARMAKOL, D-14195 BERLIN, GERMANY

来源：

BIOCHEMICAL JOURNAL | 1994年 / 304卷

关键词：

D O I：

10.1042/bj3040683

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of similar to 1 mu mol of citrulline.min(-1).mg of protein(-1) and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H(4)biopterin), FAD and FMN per mol of subunit respectively.

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页码：683 / 686

页数：4

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