DETERMINATION OF AMINOPHENOL ISOMERS BY HIGH-SPEED LIQUID-CHROMATOGRAPHY

The simultaneous quantitation of aminophenol isomers (p-, m- and o-aminophenol) and aniline, which with previously reported methods was difficult and/or complicated, has been performed using a high-speed liquid chromatographic method. The resolution of chromatograms were appropriate for separation and quantitation, when analysis times were 20 minutes. Since the method is applicable in aqueous media, a successful quantitation of p- and o-aminophenol formed by cytochrome P-450 model systems was carried out.