SOLUBILIZATION OF MEMBRANE-BOUND MATRIX-INDUCED ALKALINE-PHOSPHATASE WITH POLYOXYETHYLENE 9-LAURYL ETHER (POLIDOCANOL) - PURIFICATION AND METALLOENZYME PROPERTIES

被引：35

作者：

CIANCAGLINI, P ^{[1
]}

PIZAURO, JM ^{[1
]}

REZENDE, AA ^{[1
]}

REZENDE, LA ^{[1
]}

LEONE, FA ^{[1
]}

机构：

[1] UNIV NACL ESTADUAL SAO PAULO,FAC CIENCIAS AGRARIAS & VET,DEPT TECNOL,BR-14870 JABOTICABAL,SP,BRAZIL

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1990年 / 22卷 / 04期

关键词：

D O I：

10.1016/0020-711X(90)90141-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Matrix-induced alkaline phosphatase prepared from rat osseous plate was solubilized with polidocanol and purified on a Sephacryl S-300 column. 2. 2. Purified solubilized alkaline phosphatase has a molecular weight of ca 115,000 and bind one magnesium and two zinc ions. At least 110 detergent molecules are bound to each enzyme molecule. 3. 3. Solubilization and purification procedures did not destroy the ability of the enzyme to hydrolyze adenosine-5'-triphosphate, p-nitrophenylphosphate, pyrophosphate and bis p-nitrophenylphosphate. 4. 4. Magnesium, manganese and cobalt ions are stimulators of PNPPase activity of solubilized enzyme whereas calcium and zinc ions are inhibitors. © 1990.

引用

页码：385 / &

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