ELECTROCHEMICAL MEASUREMENT OF RELEASE OF DOPAMINE AND 5-HYDROXYTRYPTAMINE FROM SYNAPTOSOMES

被引：26

作者：

DAYTON, MA ^{[1
]}

GEIER, GE ^{[1
]}

WIGHTMAN, RM ^{[1
]}

机构：

[1] INDIANA UNIV,PROGRAM MED SCI,BLOOMINGTON,IN 47401

来源：

LIFE SCIENCES | 1979年 / 24卷 / 10期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0024-3205(79)90342-4

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Liquid chromatography with electrochemical detection has been used to determine endogenous dopamine (DA) and serotonin (5-HT) in a striatal crude synaptosomal fraction isolated by centrifugal methods. The electrochemical determinations are accomplished with a new type of electrode material, pressure annealed pyrolytic graphite. The analysis scheme permits direct quantitation of DA and 5-HT with minimal sample pretreatment. The distribution of endogenous 5-HT in each subfraction throughout the centrifugation procedure is found to be approximately parallel to that of endogenous DA. The release of DA and 5-HT from the crude synaptosome preparation exhibits the properties expected for stimulus-coupled, depolarization-induced release. The release of both neurotransmitters is Ca2+ dependent and is induced by high K+ or veratridine in the external medium. The latter is blocked by tetrodotoxin. Release is independent of ascorbic acid, but is dependent on the temperature of incubation. © 1979.

引用

页码：917 / 924

页数：8

共 21 条

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ARNOLD EB, 1977, J PHARMACOL EXP THER, V202, P544

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