AN ELECTRON-PARAMAGNETIC RESONANCE PROBE TO DETECT LOCAL Z-DNA CONFORMATIONS

被引：12

作者：

STROBEL, OK ^{[1
]}

KEYES, RS ^{[1
]}

BOBST, AM ^{[1
]}

机构：

[1] UNIV CINCINNATI,DEPT CHEM,CINCINNATI,OH 45221

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91027-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present spectroscopic evidence for an electron paramagnetic resonance (EPR) probe to detect local Z-DNA conformations in synthetic DNA. A spin labeled deoxycytidine-5′-triphosphate (pppDCAT) was co-polymerized with Micrococcus luteus DNA polymerase to yield the spin active alternating co-polymer (dG-dC,DCAT)n. The EPR spectrum of (dG-dC,DCAT)n in the Z-DNA conformation indicates a decrease in the local base dynamics by about a factor of two as compared to that computed for B-DNA. A control experiment conducted with (dA-dT, DUAT)n under similar salt conditions rules out the possibilty of observing salt induced artifacts. © 1990.

引用

页码：1435 / 1440

页数：6

共 25 条

[1] TORSION DYNAMICS AND DEPOLARIZATION OF FLUORESCENCE OF LINEAR MACROMOLECULES .1. THEORY AND APPLICATION TO DNA [J].