FLOW-INJECTION IMMUNOANALYSIS FOR THE ONLINE MONITORING OF MONOCLONAL-ANTIBODIES

被引：30

作者：

STOCKLEIN, W ^{[1
]}

SCHMID, RD ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH GMBH,MASCHERODER WEG 1,W-3300 BRAUNSCHWEIG,GERMANY

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 234卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)83541-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection immunoanalysis method is described that allows the on-line monitoring of monoclonal antibodies in the course of a hybridoma cell fermentation. Immunoreactors were developed containing either membranes or magnetic particles as the solid phase for the bound antibodies. The detection range in the various procedures described is between 1 and 100 μg ml- mouse immunoglobulin G, which is the range of interest in hybridoma cell fermentation. © 1990.

引用

页码：83 / 88

页数：6

共 21 条

[1]

ALLAIN P, 1989, CLIN CHEM, V35, P469

[2]

BOITIEUX JL, 1989, CLIN CHEM, V35, P1026

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] MONOCLONAL-ANTIBODIES INTO THE 90S - THE ALL-PURPOSE TOOL [J].

CARLSSON, R ;

GLAD, C ;

BORREBAECK, CKA .

BIO-TECHNOLOGY, 1989, 7 (06) :567-&

[5] RAPID HETEROGENEOUS COMPETITIVE ELECTROCHEMICAL IMMUNOASSAY FOR IGG IN THE PICOMOLE RANGE [J].

DEALWIS, U ;

WILSON, GS .

ANALYTICAL CHEMISTRY, 1987, 59 (23) :2786-2789

[6]

DEALWIS WU, 1985, ANAL CHEM, V57, P2754

[7]

DURST RA, 1988, CLIN CHEM, V34, P1700

[8]

HABERER K, 1988, WASSER, V71, P231

[9] ENZYME IMMUNOSENSOR USING HAPTEN-BOUND MEMBRANE AND FAB'-BETA-D-GALACTOSIDASE COMPLEXES [J].

HAGA, M ;

IKUTA, M ;

KATO, Y ;

SUZUKI, Y .

CHEMISTRY LETTERS, 1984, (08) :1313-1316

[10]

HUBER SJ, 1985, Z PFLANZENK PFLANZEN, V92, P147

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