UTILIZATION OF ZEOLITE-Y IN THE REMOVAL OF ANIONIC, CATIONIC AND NONIONIC DETERGENTS DURING PURIFICATION OF PROTEINS

被引：14

作者：

BLUM, Z ^{[1
]}

ERIKSSON, H ^{[1
]}

机构：

[1] UNIV LUND,CTR CHEM,DEPT BIOTECHNOL,S-22100 LUND,SWEDEN

来源：

BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 01期

关键词：

D O I：

10.1007/BF00152755

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrophobic zeolite Y was used to adsorb detergents from protein solutions and within one minute the commonly used detergents sodium dodecyl sulfate, cetyl trimethyl ammonium bromide, and Triton X-100 at concentrations of 10 mg/ml were adsorbed to a level below their critical micelle concentrations. From the detergent depleted solutions 77 to 85 % of the proteins were recovered; the lower value was obtained with protein concentration below one mg/ml.

引用

页码：49 / 54

页数：6