STEREOSPECIFIC ASSIGNMENTS AND CHI-1 ROTAMERS FOR FKBP WHEN BOUND TO ASCOMYCIN FROM 3JH-ALPHA,H-BETA AND 3JN,H-BETA COUPLING-CONSTANTS

被引：27

作者：

XU, RX ^{[1
]}

OLEJNICZAK, ET ^{[1
]}

FESIK, SW ^{[1
]}

机构：

[1] ABBOTT LABS,DIV PHARMACEUT DISCOVERY,D-47G,AP9,ABBOTT PK,IL 60064

来源：

FEBS LETTERS | 1992年 / 305卷 / 02期

关键词：

FKBP; ASCOMYCIN; COUPLING CONSTANT; NMR;

D O I：

10.1016/0014-5793(92)80881-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3J(H-alpha,H-beta) and 3J(N,H-beta) coupling constants were measured for isotopically labeled FKBP when bound to the immunosuppressant, ascomycin, using a H-1-coupled 3D HCCH-TOCSY and N-15-coupled 3D HSQC-TOCSY experiment, respectively. From an analysis of these two sets of coupling constants, stereospecific beta-proton assignments and chi-1 rotamers for FKBP have been obtained. All of the chi-1 rotamers were consistent with the chi-1 angles measured in the X-ray crystal structure of the FKBP/FK506 complex, suggesting that the structures of the two complexes are similar.

引用

页码：137 / 143

页数：7

共 26 条

[1] ARAI T, 1962, J ANTIBIOT, V15, P231
[2] H-1-H-1 CORRELATION VIA ISOTROPIC MIXING OF C-13 MAGNETIZATION, A NEW 3-DIMENSIONAL APPROACH FOR ASSIGNING H-1 AND C-13 SPECTRA OF C-13-ENRICHED PROTEINS
BAX, A
CLORE, GM
GRONENBORN, AM
[J]. JOURNAL OF MAGNETIC RESONANCE, 1990, 88 (02): : 425 - 431
[3] BYSTROV VF, 1976, PROGR NMR SPECTROSCO, V10, P41
[4] STRUCTURES OF LARGER PROTEINS IN SOLUTION - 3-DIMENSIONAL AND 4-DIMENSIONAL HETERONUCLEAR NMR-SPECTROSCOPY
CLORE, GM
GRONENBORN, AM
[J]. SCIENCE, 1991, 252 (5011) : 1390 - 1399
[5] H-1-N-15 SPIN-SPIN COUPLINGS IN ALUMICHROME
DEMARCO, A
LLINAS, M
WUTHRICH, K
[J]. BIOPOLYMERS, 1978, 17 (11) : 2727 - 2742
[6] EDALJI R, 1992, IN PRESS J PROT CHEM
[7] ELUCIDATION OF AMINO-ACID SPIN SYSTEMS IN PROTEINS AND DETERMINATION OF HETERONUCLEAR COUPLING-CONSTANTS BY CARBON PROTON PROTON 3-DIMENSIONAL NMR
EDISON, AS
WESTLER, WM
MARKLEY, JL
[J]. JOURNAL OF MAGNETIC RESONANCE, 1991, 92 (02): : 434 - 438
[8] ACCURATE MEASUREMENTS OF PROTON SCALAR COUPLING-CONSTANTS USING C-13 ISOTROPIC MIXING SPECTROSCOPY
EMERSON, SD
MONTELIONE, GT
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (01) : 354 - 356
[9] A METHOD FOR MEASURING H-1 H-1 COUPLING-CONSTANTS IN C-13-LABELED MOLECULES
GEMMECKER, G
FESIK, SW
[J]. JOURNAL OF MAGNETIC RESONANCE, 1991, 95 (01): : 208 - 213
[10] CLEAN TOCSY FOR H-1 SPIN SYSTEM-IDENTIFICATION IN MACROMOLECULES
GRIESINGER, C
OTTING, G
WUTHRICH, K
ERNST, RR
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (23) : 7870 - 7872

← 1 2 3 →