LEC15 CELLS TRANSFER GLUCOSYLATED OLIGOSACCHARIDES TO PROTEIN

被引:13
作者
STOLL, J
CACAN, R
VERBERT, A
KRAG, SS
机构
[1] JOHNS HOPKINS UNIV, SCH HYG & PUBL HLTH, DEPT BIOCHEM, 615 N WOLFE ST, BALTIMORE, MD 21205 USA
[2] UNIV SCI & TECHNOL LILLE, CHIM BIOL LAB, CNRS, UMR 111, F-59655 VILLENEUVE DASCQ, FRANCE
关键词
D O I
10.1016/0003-9861(92)90268-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
B4-2-1 cells (Lec15 cells) are Chinese hamster ovary cells deficient in mannosylphosphoryldolichol synthase activity. They synthesize the truncated lipid intermediate Man5GlcNAc2-P-P-dolichol rather than the Glc3Man9GlcNAc2-P-P-dolichol synthesized by wild-type cells. In this report we present evidence that these cells did synthesize glucosylated Man5GlcNAc2-P-P-dolichol, but this species represented only a minor fraction of the labeled oligosaccharide-lipid. On the other hand, glucosylated oligosaccharides were a major species transferred to protein in these cells, showing that in vivo, glucosylated oligosaccharides are preferentially transferred to protein. The truncated oligosaccharides found in B4-2-1 cells were removed from the protein by N-glycanase treatment, since they were resistant to both endo-β-N-acetylglucosaminidase H and F activity. B4-2-1 cells processed the glucosylated, truncated oligosac-charides transferred to G protein of vesicular stomatitis virus, leading to infectious virus. © 1992.
引用
收藏
页码:225 / 231
页数:7
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