CRYSTALLIZATION OF 2 BACTERIAL ENZYMES ON AN UNMANNED SPACE MISSION

被引：21

作者：

HILGENFELD, R ^{[1
]}

LIESUM, A ^{[1
]}

STORM, R ^{[1
]}

PLAASLINK, A ^{[1
]}

机构：

[1] INTOSPACE GMBH,W-3000 HANNOVER,GERMANY

来源：

JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期

关键词：

D O I：

10.1016/0022-0248(92)90265-K

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

We have grown crystals of two bacterial enzymes, thermolysin from Bacillus thermoproteolyticus and a novel 23 kD lysozyme from Streptomyces coelicolor, on the unmanned COSIMA-2 space mission. The crystals obtained under microgravity conditions were compared with crystals grown in a ground control experiment using identical hardware, and with those obtained in 'hanging drops' in the laboratory. For both enzymes, the space-grown crystals tended to be longer but thinner than their terrestrial counterparts. The diffraction properties of each type of crystals were assessed by collecting nearly complete diffraction data sets using an area detector. Space-grown crystals of the two enzymes showed much weaker diffraction than both the ground control and the laboratory-grown crystals. Possible reasons for this are discussed.

引用

页码：330 / 336

页数：7

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