PREPARATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST MYCOPLASMA-BOVIS

被引：15

作者：

BERTHOLD, E ^{[1
]}

HELLER, M ^{[1
]}

PFUTZNER, H ^{[1
]}

LEIRER, R ^{[1
]}

SACHSE, K ^{[1
]}

机构：

[1] BUNDESGESUNDHEITSAMT,INST VET MED,ROBERT VON OSTERTAG INST,BACTERIAL ANIM DIS RES INST,O-6909 JENA 9,GERMANY

来源：

JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 1992年 / 39卷 / 05期

关键词：

D O I：

10.1111/j.1439-0450.1992.tb01180.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Monoclonal antibodies (mabs) against Mycoplasma (M.) bovis were prepared for use in diagnosis of bovine mastitis. From the original 32 hybridomas actively secreting mabs against M. bovis, 6 stable lines were cloned. Two of them, Mb 5D8 and Mb 4F6, recognized M. bovis antigens of estimated molecular weights of 33 and 26kDa, respectively. They showed no cross-reaction to other bovine mycoplasmas, thus rendering them useful for specific detection of this pathogen. All mabs investigated cross-reacted with M. agalactiae which is known to be closely related to M. bovis, but does not occur in cattle. Two other mabs, Mb 5D4 and Mb 1F6, exhibited further cross-reactions to a number of bovine mycoplasma species. Finally, mabs Mb 5D5 and Mb 2G5 reacted with all mycoplasmas tested. The possibility that they recognized constituents of the broth culture medium is discussed.

引用

页码：353 / 361

页数：9

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