EXPRESSION OF HIV-1 NEF IN YEAST - THE 27-KDA NEF PROTEIN IS MYRISTYLATED AND FRACTIONATES WITH THE NUCLEUS

被引：15

作者：

MACREADIE, IG ^{[1
]}

WARD, AC ^{[1
]}

FAILLA, P ^{[1
]}

GRGACIC, E ^{[1
]}

MCPHEE, D ^{[1
]}

AZAD, AA ^{[1
]}

机构：

[1] FAIRFIELD HOSP, MACFARLANE BURNET CTR MED RES, FAIRFIELD, VIC, AUSTRALIA

来源：

YEAST | 1993年 / 9卷 / 06期

关键词：

HIV-1; NEF; SACCHAROMYCES-CEREVISIAE; YEAST EXPRESSION; MYRISTYLATION; CUP1;

D O I：

10.1002/yea.320090602

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nefgene of human immunodeficiency virus type 1 (HIV-1) has been expressed in the yeast Saccharomyces cerevisiae to produce native Nef proteins. The proteins of M(r) 27 kDa and 25 kDa, produced by translation from the first and second start codons of the nef gene react with human HIV-1 antisera. Under low-level steady-state expression conditions, Nef27 undergoes myristylation and is targeted to the nuclear fraction while Nef25 is not myristylated and not nuclear localized. When produced rapidly and to high levels, Nef27 is initially present in the cytoplasm as a soluble myristylated protein that later fractionates with the nucleus.

引用

页码：565 / 573

页数：9

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