REMOVAL OF UV-INDUCED DNA LESIONS IN MOUSE EPIDERMIS SOON AFTER IRRADIATION

被引：48

作者：

VINK, AA ^{[1
]}

HENEGOUWEN, JBAB ^{[1
]}

NIKAIDO, O ^{[1
]}

BAAN, RA ^{[1
]}

ROZA, L ^{[1
]}

机构：

[1] KANAZAWA UNIV,FAC PHARMACEUT SCI,DIV RADIAT BIOL,KANAZAWA 920,JAPAN

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1994年 / 24卷 / 01期

关键词：

UV-B; HAIRLESS MICE; CYCLOBUTANE THYMINE DIMERS; 6-4)PHOTOPRODUCTS; IMMUNOSLOTBLOT ASSAY;

D O I：

10.1016/1011-1344(94)07005-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Induction and removal of cyclobutane thymine dimers and (6-4)photoproducts were studied in epidermal DNA isolated from UV-exposed hairless mice. For the detection of DNA damage, lesion-specific monoclonal antibodies were used in an immunoslotblot assay. Following the exposure of mice to 3.0 kJ m(-2) UV-B, substantial removal of both thymine dimers (66%) and (6-4)photoproducts (77'%) was observed at 24 h after irradiation. No removal, however, was detected at 4 h after irradiation. In contrast, immunofluorescence data obtained previously showed a rapid initial dimer removal after irradiation with 1.0 kJ m(-2) UV-B (A.A. Vink, R.J.W. Berg, F.R. De Gruijl, L. Roza and R.A. Baan, Carcinogenesis, 12 (1991) 861-864.) Reinvestigation of the removal of dimers and (6-4)photoproducts shortly after three different UV doses showed a rapid decrease of both lesions at 2 h after irradiation with 1.0 W m(-2). The results obtained after irradiations with 2.0 and 3.0 kJ m(-2) UV-B suggest a saturation of repair already at 2.0 kJ m(-2). Cyclobutane dimers were found to be removed at a lower rate than (6-4)photoproducts.

引用

页码：25 / 31

页数：7

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