STUDIES ON DENITRIFICATION .8. PRODUCTION OF NITRIC OXIDE BY DENITRIFYING REACTION IN PRESENCE OF TETRAMETHYL-P-PHENYLENEDIAMINE

被引:42
作者
MIYATA, M
MORI, T
机构
[1] Biological Institute, Faculty of Science, Nagoya University, Nagoya
关键词
D O I
10.1093/oxfordjournals.jbchem.a128967
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. TMPD reduces nitrite to nitric oxide through cytochrome c-552 (denitrifying bacteria) in the presence of denitrifying enzyme. The optimal pH is 6.4-6.6.2. Carbon monoxide combines with the denitrifying enzyme itself and inhibits its activity; the inhibition is not relieved by light. There occurs a competition for the denitrifying enzyme between CO and NO produced by the denitrification.3. Nitric oxide production is strongly inhibited by KCN but not by NaN8 in the presence of TMPD. Effects of various other inhibitors are also investigated.4. By these results the possibility that NO may be an intermediate in the denitrifying reaction was reinforced.5. A possible scheme for the process of denitrification was proposed. © 1968 by the Journal of Biochemistry.
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页码:849 / &
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