CHARACTERIZATION OF A CHEMICALLY SYNTHESIZED RNA HAVING THE SEQUENCE OF THE YEAST INITIATOR TRANSFER RNAMET

被引：9

作者：

BRATTY, J

WU, TF

NICOGHOSIAN, K

OGILVIE, KK

PERREAULT, JP

KEITH, G

CEDERGREN, R

机构：

[1] UNIV MONTREAL,DEPT BIOCHIM,MONTREAL H3C 3J7,QUEBEC,CANADA

[2] MCGILL UNIV,DEPT CHEM,MONTREAL H3A 2K6,QUEBEC,CANADA

[3] CNRS,INST BIOL MOLEC & CELLULAIRE,F-67084 STRASBOURG,FRANCE

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Aminoacylation; Chemical synthesis; Purification; RNA;

D O I：

10.1016/0014-5793(90)81118-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNAMetsample and threonylated to 76 % of the level observed with native tRNAfMet from E. coli. © 1990.

引用

页码：60 / 64

页数：5

共 18 条

[1] REACTIONS AT TERMINI OF TRANSFER-RNA WITH T4 RNA LIGASE [J].