ENZYME-CATALYZED GLYCOSYLATION OF PEPTIDES USING A SYNTHETIC LIPID DISACCHARIDE SUBSTRATE

被引：44

作者：

LEE, J ^{[1
]}

COWARD, JK ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1992年 / 57卷 / 15期

关键词：

D O I：

10.1021/jo00041a015

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A lipid disaccharide, consisting of chitobiose linked to dilichol via an alpha-1-pyrophosphate, has been synthesized for use as a substrate in the enzyme-catalyzed glycosylation of peptides. For the purpose of confirming the structure of the reaction product, the expected glycopeptide was synthesized via an unambiguous, convergent method. Chromatographic and spectral comparison of the synthetic vs biosynthetic glycopeptides showed that they were identical. Thus, glycosylation of synthetic peptides by a synthetically accessible lipid disaccharide can be effected using oligosaccharyltransferase isolated from yeast.

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页码：4126 / 4135

页数：10

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