ELECTROSPRAY MASS-SPECTROMETRY OF NEUTRAL AND ACIDIC OLIGOSACCHARIDES - METHYLATED CYCLODEXTRINS AND IDENTIFICATION OF UNKNOWNS DERIVED FROM FRUIT MATERIAL

被引：27

作者：

TINKE, AP

VANDERHOEVEN, RAM

NIESSEN, WMA

VANDERGREEF, J

VINCKEN, JP

SCHOLS, HA

机构：

[1] LEIDEN AMSTERDAM CTR DRUG RES,DIV ANALYT CHEM,POB 9502,2300 RA LEIDEN,NETHERLANDS

[2] WAGENINGEN UNIV AGR,DEPT FOOD CHEM,6703 HD WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 647卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)83408-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A qualitative study of the characteristics in the mass spectrometric analysis of various neutral and acidic oligosaccharides using electrospray ionization is reported. Experiments were performed in both positive- and negative-ion modes. In the positive-ion mode molecular mass information for neutral non-derivatized oligosaccharides could be obtained UP to M(r) 4000. The method was applied to the determination of the degree of methylation of methylated beta-cyclodextrins and the identification of unknown oligosaccharides enzymatically derived from pear and apple fruit material.

引用

收藏

页码：279 / 287

页数：9

相关论文

共 30 条

[1] COMBINED LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY .2. TECHNIQUES AND MECHANISMS OF THERMOSPRAY [J].

ARPINO, P .

MASS SPECTROMETRY REVIEWS, 1990, 9 (06) :631-669

[2] THE CELLULASE OF TRICHODERMA-VIRIDE - PURIFICATION, CHARACTERIZATION AND COMPARISON OF ALL DETECTABLE ENDOGLUCANASES, EXOGLUCANASES AND BETA-GLUCOSIDASES [J].

BELDMAN, G ;

SEARLEVANLEEUWEN, MF ;

ROMBOUTS, FM ;

VORAGEN, FGJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 146 (02) :301-308

[3] ENHANCEMENT OF STRUCTURAL INFORMATION IN FAB IONIZED CARBOHYDRATE SAMPLES BY NEUTRAL GAS COLLISION [J].

CARR, SA ;

REINHOLD, VN ;

GREEN, BN ;

HASS, JR .

BIOMEDICAL MASS SPECTROMETRY, 1985, 12 (06) :288-295

[4] THE CONSTITUTION OF A PEAR CELL-WALL XYLAN [J].

CHANDA, SK ;

HIRST, EL ;

PERCIVAL, EGV .

JOURNAL OF THE CHEMICAL SOCIETY, 1951, (MAY) :1240-1246

[5] HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY COUPLED WITH MASS-SPECTROMETRY FOR THE DETERMINATION OF CARBOHYDRATES [J].

CONBOY, JJ ;

HENION, J .

BIOLOGICAL MASS SPECTROMETRY, 1992, 21 (08) :397-407

[6] PURIFICATION AND PROPERTIES OF AN ENDO-1,4-XYLANASE EXCRETED BY A HYDROLYTIC THERMOPHILIC ANAEROBE, CLOSTRIDIUM-THERMOLACTICUM - A PROPOSAL FOR ITS ACTION MECHANISM ON LARCHWOOD 4-O-METHYLGLUCURONOXYLAN [J].

DEBEIRE, P ;

PRIEM, B ;

STRECKER, G ;

VIGNON, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 187 (03) :573-580

[7] STRUCTURE DETERMINATION OF CARBOHYDRATES AND GLYCOSPHINGOLIPIDS BY FAST ATOM BOMBARDMENT MASS-SPECTROMETRY [J].

DELL, A ;

OATES, JE ;

MORRIS, HR ;

EGGE, H .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1983, 46 (JAN) :415-418

[8]

DELL A, 1983, CARBOHYD RES, V15, P41

[9] CARBOHYDRATE ANALYSIS OF WATER-SOLUBLE URONIC ACID-CONTAINING POLYSACCHARIDES WITH HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY USING METHANOLYSIS COMBINED WITH TFA HYDROLYSIS IS SUPERIOR TO 4 OTHER METHODS [J].

DERUITER, GA ;

SCHOLS, HA ;

VORAGEN, AGJ ;

ROMBOUTS, FM .

ANALYTICAL BIOCHEMISTRY, 1992, 207 (01) :176-185

[10] CHARACTERIZATION OF N-LINKED OLIGOSACCHARIDES BY ELECTROSPRAY AND TANDEM MASS-SPECTROMETRY [J].

DUFFIN, KL ;

WELPLY, JK ;

HUANG, E ;

HENION, JD .

ANALYTICAL CHEMISTRY, 1992, 64 (13) :1440-1448