IMPROVED BAND SHIFT ASSAY FOR THE SIMULTANEOUS ANALYSIS OF PROTEIN DNA INTERACTIONS AND ENZYMATIC FUNCTIONS OF DNA-POLYMERASES

被引：5

作者：

STRICK, R ^{[1
]}

KNOPF, CW ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,ANGEW TUMORVIROL,NEUENHEIMER FELD 280,W-6900 HEIDELBERG 1,GERMANY

来源：

FEBS LETTERS | 1992年 / 300卷 / 02期

关键词：

DNA POLYMERASE; PROTEIN-DNA INTERACTION; EXONUCLEOLYTIC ACTIVITY; 3'-]5'; ENZYMATIC ASSAY; GEL ELECTROPHORESIS;

D O I：

10.1016/0014-5793(92)80182-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method to assay the major properties of DNA polymerases such as template binding, polymerase and exonuclease activities in one step is exemplified with the DNA polymerases of E. coli, bacteriophage T4 and herpes simplex virus. Combining the advantages of the band-shift assay with the resolving power of polyacrylamide gradient gel electrophoresis, the procedure is particularly useful for a rapid functional analysis of mutant polymerases as well as inhibitors of DNA replication.

引用

页码：141 / 144

页数：4

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