PURIFICATION, CHARACTERIZATION AND CRYSTALLIZATION OF HUMAN PLATELET PROFILIN EXPRESSED IN ESCHERICHIA-COLI

被引：47

作者：

FEDOROV, AA

POLLARD, TD

ALMO, SC

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT BIOCHEM,BRONX,NY 10461

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT CELL BIOL & ANAT,BALTIMORE,MD 21205

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 241卷 / 03期

关键词：

PROFILIN; CYTOSKELETON; CRYSTALLIZATION; EXPRESSION; PROTEIN STRUCTURE;

D O I：

10.1006/jmbi.1994.1522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human platelet profilin was expressed in Escherichia coli using a T7 based expression vector. The recombinant material is similar to authentic human platelet profilin based on the measured K-d, for rabbit skeletal muscle actin. Crystals of the recombinant material were obtained from both PEG 8000 and (NH4)(2)SO4. These crystals are isomorphous and belong to the monoclinic space group C2, a=75.0, b=32.0, c=62.5 5, beta=123 degrees. These crystals contain one molecule in the asymmetric unit and diffract to at least 2.0 Angstrom.

引用

页码：480 / 482

页数：3

共 25 条

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