MOLECULAR-CLONING AND MAPPING OF 10 NEW PROBES ON THE HUMAN-Y CHROMOSOME

被引：32

作者：

NAKAHORI, Y

TAMURA, T

NAGAFUCHI, S

FUJIEDA, K

MINOWADA, S

FUKUTANI, K

FUSE, H

HAYASHI, K

KUROKI, Y

FUKUSHIMA, Y

AGEMATSU, K

KUNO, T

KANEKO, S

YAMADA, K

KITAGAWA, T

NONOMURA, M

FUKUDA, S

KUSANO, M

ONIGATA, S

HIBI, I

NAKAGOME, Y

机构：

[1] NATL CHILDRENS MED RES CTR, DEPT CONGENITAL ABNORMALITIES RES, 3-35-31 TAISHIDO, SETAGAYA KU, TOKYO 154, JAPAN

[2] KYORIN UNIV, SCH HLTH SCI, DEPT CLIN GENET, TOKYO, JAPAN

[3] HOKKAIDO UNIV, SCH MED, DEPT PEDIAT, SAPPORO, HOKKAIDO 060, JAPAN

[4] UNIV TOKYO, SCH MED, DEPT UROL, TOKYO 113, JAPAN

[5] ASOKA HOSP, DEPT UROL, TOKYO, JAPAN

[6] TOYAMA MED & PHARMACEUT UNIV, DEPT UROL, TOMYAMA, JAPAN

[7] KYOTO UNIV, SCH MED, DEPT OBSTET & GYNECOL, KYOTO 606, JAPAN

[8] KANAGAWA CHILDRENS MED CTR, DIV MED GENET, YOKOHAMA, KANAGAWA, JAPAN

[9] SAITAMA CHILDRENS MED CTR, IWATSUKI, SAITAMA, JAPAN

[10] SHINSHU UNIV, SCH MED, DEPT PEDIAT, MATSUMOTO, NAGANO 390, JAPAN

[11] SAGA MED UNIV, DEPT PEDIAT, SAGA, JAPAN

[12] KEIO UNIV, SCH MED, DEPT OBSTET & GYNECOL, TOKYO 108, JAPAN

[13] NATL MED CTR HOSP, DEPT GENET, TOKYO, JAPAN

[14] NIHON UNIV, SCH MED, DEPT PEDIAT, TOKYO 101, JAPAN

[15] KYOTO UNIV, SCH MED, DEPT UROL, KYOTO 606, JAPAN

[16] ST MARYS HOSP, KURUME, JAPAN

[17] GUNMA UNIV, SCH MED, DEPT INTERNAL MED, MAEBASHI, GUNMA 371, JAPAN

[18] TOMIOKA KOSEI HOSP, DEPT PEDIAT, TOMIOKA, GUNMA, JAPAN

[19] NATL CHILDRENS HOSP, DIV ENDOCRINOL, TOKYO 154, JAPAN

来源：

GENOMICS | 1991年 / 9卷 / 04期

关键词：

D O I：

10.1016/0888-7543(91)90373-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a novel positive cloning vector whose use precludes the cloning of any fragments less than 0.8 kb as well as 3.4-kb EcoRI fragments of DYZ1, the largest repeating-DNA family on the long arm of the human Y chromosome. Using this vector, we subcloned inserts of a Y-chromosome-specific phage library constructed from EcoRI-digested flow-sorted Y-chromosome DNA. Ten novel Y-specific fragments were obtained. Their localization on the Y chromosome was determined by deletion mapping using clinical samples with structurally abnormal Y chromosomes. The long arm of the Y chromosome was divided into 12 segments by the novel probes in combination with established probes. The amelogenin-like sequence, mapped on the long arm in Human Gene Mapping 10, has been mapped on the short arm. © 1991.

引用

页码：765 / 769

页数：5

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