CHARACTERIZATION OF LISTERIA-MONOCYTOGENES ISOLATES BY SOUTHERN BLOT HYBRIDIZATION

被引：14

作者：

WESLEY, IV ^{[1
]}

WESLEY, RD ^{[1
]}

HEISICK, J ^{[1
]}

HARRELL, F ^{[1
]}

WAGNER, D ^{[1
]}

机构：

[1] US FDA,MINNEAPOLIS,MN

来源：

VETERINARY MICROBIOLOGY | 1990年 / 24卷 / 3-4期

关键词：

D O I：

10.1016/0378-1135(90)90182-U

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A synthetic deoxyribonucleotide probe for virulent Listeria monocytogenes, designated ADO7, was evaluated for its ability to identify restriction fragments of L. monocytogenes with nucleic acid sequences homologous with the β-hemolysin gene by Southern blot hybridization of clinical and food isolates. The synthetic probe hybridized with three restriction fragments (approximately 1.1, 0.86, and 0.76 kb) of the serotype 1/2A isolates. Southern blot hybridization of the serogroup 4B isolates indicated that the nucleic acid sequences homologous with the β-hemolysin gene probe were limited to a single restriction fragment of approximately 1 kb. © 1990.

引用

收藏

页码：341 / 353

页数：13

相关论文

共 20 条

[1]

Centers for Disease Control (CDC), 1985, MMWR Morb Mortal Wkly Rep, V34, P357

[2] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[3] DETECTION OF HEMOLYTIC LISTERIA-MONOCYTOGENES BY USING DNA COLONY HYBRIDIZATION [J].

DATTA, AR ;

WENTZ, BA ;

HILL, WE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (09) :2256-2259

[4] SYNTHETIC OLIGODEOXYRIBONUCLEOTIDE PROBES FOR DETECTION OF LISTERIA-MONOCYTOGENES [J].

DATTA, AR ;

WENTZ, BA ;

SHOOK, D ;

TRUCKSESS, MW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (12) :2933-2937

[5] PASTEURIZED MILK AS A VEHICLE OF INFECTION IN AN OUTBREAK OF LISTERIOSIS [J].

FLEMING, DW ;

COCHI, SL ;

MACDONALD, KL ;

BRONDUM, J ;

HAYES, PS ;

PLIKAYTIS, BD ;

HOLMES, MB ;

AUDURIER, A ;

BROOME, CV ;

REINGOLD, AL .

NEW ENGLAND JOURNAL OF MEDICINE, 1985, 312 (07) :404-407

[6] TRANSPOSON MUTAGENESIS AS A TOOL TO STUDY THE ROLE OF HEMOLYSIN IN THE VIRULENCE OF LISTERIA-MONOCYTOGENES [J].

GAILLARD, JL ;

BERCHE, P ;

SANSONETTI, P .

INFECTION AND IMMUNITY, 1986, 52 (01) :50-55

[7] AN OUTBREAK OF TYPE-4B LISTERIA-MONOCYTOGENES INFECTION INVOLVING PATIENTS FROM 8 BOSTON HOSPITALS [J].

HO, JL ;

SHANDS, KN ;

FRIEDLAND, G ;

ECKIND, P ;

FRASER, DW .

ARCHIVES OF INTERNAL MEDICINE, 1986, 146 (03) :520-524

[8] HEMOLYSIN SUPPORTS SURVIVAL BUT NOT ENTRY OF THE INTRACELLULAR BACTERIUM LISTERIA-MONOCYTOGENES [J].

KUHN, M ;

KATHARIOU, S ;

GOEBEL, W .

INFECTION AND IMMUNITY, 1988, 56 (01) :79-82

[9]

KVENBERG JE, 1988, MICROBIOL SCI, V5, P355

[10] EPIDEMIC LISTERIOSIS ASSOCIATED WITH MEXICAN-STYLE CHEESE [J].

LINNAN, MJ ;

MASCOLA, L ;

LOU, XD ;

GOULET, V ;

MAY, S ;

SALMINEN, C ;

HIRD, DW ;

YONEKURA, ML ;

HAYES, P ;

WEAVER, R ;

AUDURIER, A ;

PLIKAYTIS, BD ;

FANNIN, SL ;

KLEKS, A ;

BROOME, CV .

NEW ENGLAND JOURNAL OF MEDICINE, 1988, 319 (13) :823-828