DETERMINATION OF CHIRAL REAGENT PURITY BY CAPILLARY ELECTROPHORESIS

被引：24

作者：

ENGSTROM, A ^{[1
]}

WAN, H ^{[1
]}

ANDERSSON, PE ^{[1
]}

JOSEFSSON, B ^{[1
]}

机构：

[1] UNIV STOCKHOLM,ARRHENIUS LABS NAT SCI,DEPT ANALYT CHEM,S-10691 STOCKHOLM,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 715卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00588-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the determination of the enantiomeric purity of the chiral reagent 1-(9-fluorenyl)ethyl chloroformate (FLEC) has been developed. The reagent is reacted with glycine, an achiral compound, and separated by capillary electrophoresis using beta- or gamma-cyclodextrin as chiral selectors. A general equation for the recalculation of the measured values with respect to the chiral reagent purity is presented. The suitability of this approach is practised on the peptide D-Arginine-Glycine to determine trace amounts of the enantiomeric contaminant, the L-form.

引用

页码：151 / 158

页数：8

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