A PCR-BASED LINKAGE MAP OF HUMAN CHROMOSOME-1

被引：50

作者：

ENGELSTEIN, M

HUDSON, TJ

LANE, JM

LEE, MK

LEVERONE, B

LANDES, GM

PELTONEN, L

WEBER, JL

DRACOPOLI, NC

机构：

[1] MIT,DEPT BIOL,CTR GENOME RES,E17-121,CAMBRIDGE,MA 02139

[2] NATL PUBL HLTH INST,SF-00280 HELSINKI 28,FINLAND

[3] INTEGRAT GENET INC,DEPT HUMAN GENET,FRAMINGHAM,MA 01701

[4] MARSHFIELD CLIN FDN MED RES & EDUC,MARSHFIELD,WI 54449

来源：

GENOMICS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1006/geno.1993.1054

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A genetic linkage map of human chromosome 1 based entirely on PCR-typable markers has been developed using 38 simple sequence repeat (SSR) polymorphisms. These SSRs include 36 dinucleotide repeats and 2 tetranucleotide repeats. The average heterozygosity at these markers was 0.73 and ranged from 0.52 to 0.95. Multipoint linkage analysis was used to develop a map of these 38 markers in which the relative placement of each locus is supported by likelihood odds =1000:1. This PCR-based map was anchored at the centromere by the D1Z5 α-satellite polymorphism, and the ends of the map were defined by D1Z2 and D1S68, which are the most distal loci in the CEPH consortium map of chromosome 1. The sex-averaged, male, and female maps extend for 328, 273, and 409 cM, respectively. The average distance between markers on the sex-averaged map is 8 cM, and the largest interval is 32 cM. This map of highly informative PCR-based markers will provide a rapid means of screening human chromosome 1 for the presence of disease genes. © 1993 Academic Press. All rights reserved.

引用

页码：251 / 258

页数：8

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