PRODUCTION OF TUMOR-NECROSIS-FACTOR-ALPHA BY RESIDENT AND ACTIVATED MURINE MACROPHAGES

被引:41
作者
TACHIBANA, K
CHEN, GJ
HUANG, DS
SCUDERI, P
WATSON, RR
机构
[1] NAGASAKI UNIV,FAC FISHERIES,DEPT FISHERY FOOD SCI,NAGASAKI 852,JAPAN
[2] UNIV ARIZONA,ARIZONA HLTH SCI CTR,DEPT FAMILY & COMMUNITY MED,TUCSON,AZ 85724
[3] MILES INC,BERKELEY,CA
关键词
TUMOR NECROSIS FACTOR; MACROPHAGES;
D O I
10.1002/jlb.51.3.251
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Alveolar macrophages (Amphis), resident peritoneal macrophages (RPmphis), and thioglycolate-elicited peritoneal macrophages (TGPmphis) were isolated from C57BL/6 mice and incubated with lipopolysaccharide (LPS), stimulated cell supernatant, or recombinant interferon gamma (IFN-gamma) for 24 h. Tumor necrosis factor (TNF) in cell-free supernatants was measured by enzyme-linked immunosorbent assay. Amophis incubated with 10(3) ng/ml LPS produced 50 times more TNF than RPmphis and 5 times more than TGPmphis, and LPS alone induced maximum TNF production by Amphis. Stimulated cell supernatant or recombinant IFN-gamma alone did not induce TNF production. A combination of LPS with stimulated cell supernatant or IFN-gamma had only a limited synergistic effect on TNF production by Amphis. However, both LPS and stimulated cell supernatant or recombinant IFN-gamma induced maximum TNF production by RPmphis and TGPmphis. TGPmphis showed greater sensitivity to LPS and stimulated cell supernatant or IFN-gamma with regard to TNF production than the other macrophage populations investigated.
引用
收藏
页码:251 / 255
页数:5
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