SPECIFIC IDENTIFICATION AND ENUMERATION OF ACETOBACTER-DIAZOTROPHICUS IN SUGARCANE

被引：58

作者：

LI, RP

MACRAE, IC

机构：

[1] Department of Microbiology, The University of Queensland

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 1992年 / 24卷 / 05期

关键词：

D O I：

10.1016/0038-0717(92)90203-A

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

An indirect ELISA specific for the identification and enumeration of Acetobacter diazotrophicus was developed. The specificity and sensitivity of the ELISA relied on the optimal dilutions of the IgG and the enzyme conjugate, and the adsorption of the IgG with Acetobacter pasteurianus. 10(6)-10(7) Acetobacter diazotrophicus cells were found to be present g-1 of dry roots, rhizosphere soils, leaves or stems of sugarcane plants. Sugarcane setts (stem cuttings used as seed pieces) and non-rhizosphere soils have much lower populations of this species which could only be detected after enrichment in semisolid sucrose medium by the ELISA. This bacterium could not be detected from grasses even after enrichment in semisolid sucrose medium.

引用

页码：413 / 419

页数：7

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