DEGRADATION OF PROTEINS FROM THYLAKOID MEMBRANES IN SENESCING WHEAT LEAVES AT HIGH-TEMPERATURE

This investigation determined whether thylakoid proteins would be degraded more rapidly or not in senescing wheat (Triticum aestivum L. em. Thell.) leaves concurrently exposed to high temperatures. Excised leaves were pulse-labelled with [S-35]-methionine for a 12 h period, and then incubated at 22, 32 or 42-degrees-C for 0, 1, 2, or 3 d, before extracting a thylakoid enriched membrane sample. After electrophoretic separation, two prominent [S-35]-labelled protein bands were chosen for further analyses. Band A contained the D-1 thylakoid protein and band B contained thylakoid proteins of the light harvesting complex (LHCII) associated with photosystem II (PSII). Total protein, [S-35]-labelled protein, band A protein, and band B protein within the thylakoid enriched membrane samples were measured. Unlabelled thylakoid enriched membrane samples, extracted from leaves given similar treatments, were used to measure uncoupled whole-chain and photosystem II (PSII) electron transport and chlorophyll fluorescence. Accentuated decline in whole-chain and PSII electron transport, increasing F(o) values, and decreasing F(max) values were a result of high temperature injury in leaves treated at 42-degrees-C. None of the thylakoid enriched membrane protein fractions were degraded more rapidly in high-temperature treated leaves. Degradation of the total [S-35]-labelled membrane proteins and band B was inhibited by the 42-degrees-C treatment. The results indicate that high temperature stress may disrupt some aspects of normal senescence.