ANALYSIS OF PARALYTIC SHELLFISH POISONING TOXINS BY AUTOMATED PRECOLUMN OXIDATION AND MICROCOLUMN LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：20

作者：

JANECEK, M

QUILLIAM, MA

LAWRENCE, JF

机构：

[1] NATL RES COUNCIL CANADA,ATLANTIC REG LAB,INST MARINE BIOSCI,1411 OXFORD ST,HALIFAX B3H 3Z1,NS,CANADA

[2] FOOD DIRECTORATE,BUR CHEM SAFETY,DIV FOOD RES,HLTH PROTECT BRANCH,OTTAWA K1A 0L2,ON,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 644卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80715-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Periodate oxidation of the toxins responsible for paralytic shellfish poisoning (PSP) yields fluorescent purines suitable for trace analysis by reversed-phase LC. Mobile phases containing perfluorinated acids, such as heptafluorobutyric acid, as ion-pair agents were found to provide high capacity factors for the oxidized products. Gradient elution on a microbore column with large volume injections and fluorescence detection permitted the detection of femtomole quantities of PSP toxins. A fully automated pre-column oxidation procedure was developed for an LC autosampler system in order to improve precision and allow unattended analyses. The complete method was applied successfully to various samples, including shellfish and toxic phytoplankton.

引用

页码：321 / 331

页数：11

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