PRODUCTION OF CATECHOLS AND MUCONIC ACIDS FROM VARIOUS AROMATICS BY THE STYRENE-DEGRADER RHODOCOCCUS-RHODOCHROUS NCIMB-13259

被引：17

作者：

WARHURST, AM

CLARKE, KF

HILL, RA

HOLT, RA

FEWSON, CA

机构：

[1] UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,SCOTLAND

[2] UNIV GLASGOW,DEPT CHEM,GLASGOW G12 8QQ,SCOTLAND

[3] SCI & TECH SERV LTD,BCRA,CHESTERFIELD S42 6NG,DERBY,ENGLAND

[4] ZENECA BIOPROD,BILLINGHAM TS23 1YN,CLEVELAND,ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 05期

关键词：

D O I：

10.1007/BF01023335

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Intact cells of Rhodococcus rhodochrous NCIMB 13259 which had been grown in the presence of styrene were used to produce metabolic intermediates from other aromatic substrates in the presence of 3-fluorocatechol as an inhibitor of catechol oxygenase. Toluene and ethylbenzene gave 3-methylcatechol and 3-ethylcatechol respectively. The enol form of 3-acetylcatechol was produced from acetophenone and 1-phenylethanol. Benzoic acid was produced from cinnamic acid. This organism cannot metabolise muconic acids, and methylmuconic acid and ethylmuconic acid accumulated under appropriate conditions. 2-Fluoromuconic acid was also produced from the added 3-fluorocatechol. This organism provides a convenient tool for producing catechols and muconic acids from a variety of substrates without need for genetic manipulation.

引用

页码：513 / 516

页数：4