ALKALI-LABILE SITES AND POSTIRRADIATION EFFECTS IN GAMMA-IRRADIATED BIOLOGICALLY-ACTIVE DOUBLE-STRANDED DNA IN AQUEOUS-SOLUTION

被引：33

作者：

LAFLEUR, MVM

WOLDHUIS, J

LOMAN, H

机构：

[1] Physics Laboratory of the Vrije Universiteit, Department of Biophysics, De Boelelaan, 1081, Amsterdam

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1979年 / 36卷 / 03期

关键词：

D O I：

10.1080/09553007914551011

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gamma-irradiation of double-stranded RF-DNA of bacteriophage φX174 in aqueous solution in the presence of oxygen produces at least one type of alkalilabile site. It is lethal and gives rise to breaks by alkali and is identical with the damage which becomes manifest by post-irradiation heat treatment. The effect of alkali is dependent on temperature. Furthermore, the excision repair system is not involved in eliminating lethal nucleotide damage in RFI-DNA. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：241 / 247

页数：7

共 16 条

[1] SINGLE-STRAND BREAKS IN GAMMA-IRRADIATED PHIX174 DNA INDUCED BY EXPOSURE TO ALKALI [J].

ACHEY, PM ;

BILLEN, D ;

BELTRANENA, HP .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE, 1971, 20 (05) :501-+

[2] RADIOSENSITIVITY OF BACTERIOPHAGE DNA IN AQUEOUS SOLUTION [J].

BLOK, J ;

LUTHJENS, LH ;

ROOS, ALM .

RADIATION RESEARCH, 1967, 30 (03) :468-&

[3] END GROUP DETERMINATION IN GAMMA-IRRADIATED DNA [J].

BOPP, A ;

HAGEN, U .

BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 209 (02) :320-&

[4] INACTIVATION OF BIOLOGICALLY-ACTIVE DNA BY RADIATION-INDUCED PHENYLALANINE RADICALS [J].

DEJONG, J ;

BLOK, J ;

LOMAN, H .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE, 1972, 22 (01) :11-&

[5] PREPARATION OF DOUBLE-STRANDED DNA (REPLICATIVE FORM) OF BACTERIOPHAGE PHIX174 - A SIMPLIFIED METHOD [J].

JANSZ, HS ;

POUWELS, PH ;

SCHIPHOR.J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 123 (03) :626-&

[6] MONOPHOSPHATE END GROUPS PRODUCED IN RADIATION-INDUCED STRAND BREAKAGE IN DNA [J].

KAY, E ;

WARD, JF .

RADIATION RESEARCH, 1977, 69 (01) :185-193

[7] RADIATION-INDUCED SINGLE-STRAND BREAKS IN DOUBLE-STRANDED CIRCULAR DNA [J].

KESSLER, B ;

BOPP, A ;

HAGEN, U .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE, 1971, 20 (01) :75-&

[8] ALKALI-LABILE SITES AND POSTIRRADIATION EFFECTS IN SINGLE-STRANDED-DNA INDUCED BY H-RADICALS [J].

LAFLEUR, MVM ;

VANHEUVEL, M ;

WOLDHUIS, J ;

LOMAN, H .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1978, 33 (03) :273-281

[9] BIOLOGICAL RELEVANCE OF GAMMA-RAY-INDUCED ALKALI-LABILE SITES IN SINGLE-STRANDED-DNA IN AQUEOUS-SOLUTIONS [J].

LAFLEUR, MVM ;

VANHEUVEL, M ;

VANDERSTROOM, HA ;

LOMAN, H .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1976, 30 (03) :223-228

[10] BIOLOGICAL RELEVANCE OF ALKALI-LABILE SITES IN DOUBLE-STRANDED DNA AFTER GAMMA-IRRADIATION [J].

LUCKEHUHLE, C .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1975, 27 (01) :1-6

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