CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDY OF CATHEPSIN-D INHIBITOR FROM POTATOES

被引:7
作者
BAUDYS, M [1 ]
GHOSH, M [1 ]
HARLOS, K [1 ]
MARES, M [1 ]
FUSEK, M [1 ]
KOSTKA, V [1 ]
BLAKE, CCF [1 ]
机构
[1] CZECHOSLOVAK ACAD SCI, INST ORGAN CHEM & BIOCHEM, CS-16610 PRAGUE 6, CZECHOSLOVAKIA
关键词
D O I
10.1016/0022-2836(91)90869-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Single crystals of the glycosylated inhibitor of cathepsin D and trypsin isolated from potato tubers were obtained using the hanging drop vapor diffusion method and ammonium nitrate as precipitant. The crystals exhibit strong F222 pseudo symmetry but belong to the orthorhombic space group C222 or C2221, with cell parameters a = 73.8 A ̊, b = 119.9 A ̊ and c = 133.2 A ̊ with two molecules per asymmetric unit. The crystals diffract to a resolution of 2.4 Å. © 1991.
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页码:21 / 22
页数:2
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