IMMUNOGOLD SILVER STAINING OF EXTRACELLULAR LIGNINASES SECRETED BY PHANEROCHAETE-CHRYSOSPORIUM

被引：16

作者：

LACKNER, R ^{[1
]}

SREBOTNIK, E ^{[1
]}

MESSNER, K ^{[1
]}

机构：

[1] VIENNA TECH UNIV,INST BIOCHEM TECHNOL & MIKROBIOL,MYKOL ABT,A-1060 VIENNA,AUSTRIA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1991年 / 37卷 / 09期

关键词：

LIGNINASE SECRETION; IMMUNOGOLD SILVER STAINING; PHANEROCHAETE-CHRYSOSPORIUM;

D O I：

10.1139/m91-113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on cover-slip cultures was observed only when the cell wall was highly porous and translocation of cell material occurred. These experiments suggested that there is a correlation between hyphal lysis and the release of extracellular ligninases from hyphae.

引用

页码：665 / 668

页数：4

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