HYBRID BIOSENSOR FOR THE DETERMINATION OF SUCROSE

被引：48

作者：

BARLIKOVA, A

SVORC, J

MIERTUS, S

机构：

[1] Department of Analytical Chemistry, Slovak Technical University, 812 37 Bratislava

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 247卷 / 01期

关键词：

AMPEROMETRY; BIOSENSORS; ENZYME REACTOR; FOODS; SUCROSE; YEAST CELL WALLS;

D O I：

10.1016/S0003-2670(00)83056-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Yeast cell walls of Saccharomyces cerevisiae as a source of invertase together with glucose oxidase were used for the construction of a hybrid sucrose sensor. The biocatalytic layer was prepared by co-immobilizing yeast cell walls and glucose oxidase on a nylon network via glutardialdehyde and was fixed to the Clark oxygen electrode. The mutarotation of alpha- to beta-glucose was accelerated by phosphate ions instead of mutarotase. The influence of pH, temperature and optimum composition of the biocatalytic layer was tested. Test samples and real samples were examined and the results were compared with those obtained by means of a glucose electrode. The sensor is stable for more than 2 months and the linear range extends to 1.3 x 10(-3) mol 1(-1).

引用

页码：83 / 87

页数：5

共 20 条

[1]

BERGMAYER HU, 1984, METHODS ENZYMATIC AN

[2] MAGNETIC ENZYME MEMBRANES AS ACTIVE ELEMENTS OF ELECTROCHEMICAL SENSORS - LACTOSE, SACCHAROSE, MALTOSE BIENZYME ELECTRODES [J].