REVERSIBLE IMMOBILIZATION OF ANTIBODIES ON MAGNETIC BEADS

被引：21

作者：

SCOUTEN, WH ^{[1
]}

KONECNY, P ^{[1
]}

机构：

[1] BAYLOR UNIV, DEPT CHEM, WACO, TX 76798 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 205卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90441-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A streptavidin-biotin system was utilized to prepare an antibody-polyadenylic acid conjugate which was subsequently attached to commercially available magnetic beads, Dynabeads oligo(dT)25. Biotinylated polyadenylic acid was combined with streptavidin and the resulting polyadenylic acid-streptavidin was conjugated with an antibody-biotin derivative. The immobilized antibody-polyadenylic acid conjugate was separated from the reaction mixture by hybridization with complementary oligonucleotide immobilized on the surface of Dynabeads oligo(dT)25. The immobilized antibody-polyadenylic acid can be released from the carrier, utilizing low-ionic-strength buffers. The system is intended to be utilized in cell sorting, using immobilized antibodies against cell surface antigens. Dissociation of antibody-containing conjugate from magnetic beads is essential for the isolation of viable cells via positive cell sorting. © 1992.

引用

页码：313 / 318

页数：6

共 29 条

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