STRUCTURE DETERMINATION OF GALACTO-OLIGOSACCHARIDES BY PYRIDYLAMINATION AND NMR-SPECTROSCOPY

被引：27

作者：

KIMURA, K

MATSUMOTO, K

ISHIHARA, C

HARADA, K

MIYAGI, A

机构：

[1] YAKULT Central Institute for Microbiological Research, Kunitachi, Tokyo, 186

来源：

CARBOHYDRATE RESEARCH | 1995年 / 270卷 / 01期

关键词：

STRUCTURE DETERMINATION; GALACTOOLIGOSACCHARIDES; PYRIDYLAMINATION;

D O I：

10.1016/0008-6215(94)00010-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Galacto-oligosaccharides formed from lactose by the action of some beta-galactosidases were subjected to gel chromatography on Bio-Gel P-2, and the resulting oligosaccharide fractions were converted into pyridylamino (PA) derivatives. Each PA-oligosaccharide fraction, which consisted of several isomers in a given size-class, was then subjected to HPLC on an ODS column. Twenty-one individual galacto-oligosaccharide components were isolated in this way. The structures of most of these compounds, namely six disaccharides, five trisaccharides, two tetrasaccharides, and a pentasaccharide, were determined by C-13-NMR spectroscopy. The results obtained will be useful for the study of the activity of various galacto-oligosaccharides on the growth of Bifidobacterium species.

引用

页码：33 / 42

页数：10

共 14 条

[1]

Tanaka, Takayama, Morotomi, Kuroshima, Ueyama, Matsumoto, Kuroda, Mutai, Effects of Administration of TOS and Bifidobacterium breve 4006 on the Human Fecal Flora, Bifidobacteria and Microflora, 2, pp. 17-24, (1983)

[2]

Ito, Deguchi, Miyamori, Matsumoto, Kikuchi, Matsumoto, Kobayashi, Yajima, Kan, Effects of Administration of Galactooligosaccharides on the Human Faecal Microflora, Stool Weight and Abdominal Sensation, Microbial Ecology in Health and Disease, 3, pp. 285-292, (1990)

[3]

Zarate, Lopetz-Leiva, J. Food Prot., 53, pp. 262-268, (1990)

[4]

Matsumoto, Kobayashi, Tamura, Watanabe, Kan, Denpun Kagaku, 36, pp. 123-130, (1989)

[5]

Toba, Yokota, Adachi, Food Chem., 16, pp. 147-162, (1985)

[6]

Prakash, Suyama, Itoh, Adachi, Structure elucidation of major galactooligosaccharides formed by growing cultures of Trichoderma harzianum, Journal of Agricultural and Food Chemistry, 37, pp. 334-337, (1989)

[7]

Hase, Ibuki, Ikenaka, J. Biochem. (Tokyo), 95, pp. 197-203, (1984)

[8]

Kondo, Suzuki, Kuraya, Hase, Kato, Ikenaka, Agric. Biol. Chem., 54, pp. 2169-2170, (1990)

[9]

Suzuki, Kondo, Kato, Hase, Ikenaka, Analysis by high-performance anion-exchange chroma-tography of component sugars as their fluorescent pyridylamino derivatives., Agricultural and Biological Chemistry, 55, pp. 283-284, (1991)

[10]

Takemoto, Hase, Ikenaka, Anal. Biochem., 145, pp. 245-250, (1985)

← 1 2 →