KINETIC CHARACTERIZATION OF A PEPTIDE INHIBITOR OF TRYPSIN ISOLATED FROM A SYNTHETIC PEPTIDE COMBINATORIAL LIBRARY

被引：5

作者：

COOMBS, GS ^{[1
]}

HAZZARD, J ^{[1
]}

COREY, DR ^{[1
]}

机构：

[1] UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST,DEPT PHARMACOL,DALLAS,TX 75235

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1995年 / 5卷 / 06期

关键词：

D O I：

10.1016/0960-894X(95)00082-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Recently Eichler and Houghten(1) have described the use of a combinatorial peptide library to optimize small trypsin inhibitors. Our kinetic analysis of the most inhibitory peptide, YYGAKIYRPDKM, reveals that it is an inefficiently cleaved substrate for trypsin due to a low k(cat), possesses a relatively low K-M, and may exhibit nonproductive binding.

引用

页码：611 / 614

页数：4

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