STRUCTURE-ANALYSIS OF BRANCHED OLIGOSACCHARIDES USING POST-SOURCE DECAY IN MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY

被引：54

作者：

SPENGLER, B ^{[1
]}

KIRSCH, D ^{[1
]}

KAUFMANN, R ^{[1
]}

LEMOINE, J ^{[1
]}

机构：

[1] UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB,F-59655 VILLENEUVE DASCQ,FRANCE

来源：

ORGANIC MASS SPECTROMETRY | 1994年 / 29卷 / 12期

关键词：

D O I：

10.1002/oms.1210291210

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) of sodium ion-attached branched oligosaccharides derived from glycoproteins was demonstrated as a method of structure analysis by reflectron time-of-flight (TOF) mass spectrometry. Mono-, di- and triantennary structures were investigated. The fragmentation patterns of these (structurally related) substances as obtained in the positive-ion mode showed characteristic differences correlated with branching sites and linkage positions. Two-bond ring cleavages as known from fast atom bombardment/collision-induced dissociation and IR laser desorption mass spectrometry were also observed. Internal fragment ions formed by up to four consecutive cleavages were obtained with high intensity, allowing the branching structure of complex carbohydrates to be identified. PSD-MALDI of oligosaccharides is characterized by high sensitivity, very good signal-to-noise ratios and high reproducibility of fragmentation patterns and signal intensities.

引用

页码：782 / 787

页数：6

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