RADIOIMMUNOASSAY FOR GAMMA-ENDORPHIN

被引：22

作者：

JEGOU, S ^{[1
]}

TONON, MC ^{[1
]}

LEBOULENGER, F ^{[1
]}

DELARUE, C ^{[1
]}

VAUDRY, H ^{[1
]}

LING, N ^{[1
]}

机构：

[1] SALK INST BIOL STUDIES,NEUROENDOCRINOL LAB,LA JOLLA,CA 92037

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1978年 / 83卷 / 01期

关键词：

D O I：

10.1016/0006-291X(78)90417-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A double antibody radioimmunoassay technique for γ-endorphin has been developed. The antisera have been raised in rabbits against synthetic γ-endorphin coupled to bovine serum albumin by carbodiimide. The best antibody has a working titer of 1 35,000 and can detect less than 9 pg of peptide. The usable range of the standard curve is between 9 to 2400 pg. This antiserum probably binds the Glu8-Leu17 region of γ-endorphin and shows only weak cross-reactivity with α-endorphin, β-endorphin and β-lipotropin. Parallelism is observed between the standard curve and the inhibition curves obtained with rat neurohypophysis-pars intermedia extracts or rat plasma. © 1978.

引用

页码：201 / 208

页数：8

共 26 条

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