DIFFERENTIATION OF MYCOBACTERIUM SPECIES BY DIRECT SEQUENCING OF AMPLIFIED DNA

被引：258

作者：

ROGALL, T ^{[1
]}

FLOHR, T ^{[1
]}

BOTTGER, EC ^{[1
]}

机构：

[1] HANOVER MED SCH,INST MED MIKROBIOL,KONSTANTY GUTSCHOW STR 8,W-3000 HANOVER 61,GERMANY

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

关键词：

D O I：

10.1099/00221287-136-9-1915

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Nucleotide sequences specific for a range of Mycobacterium species were defined by computer-assisted sequence comparisons of small subunit ribosomal RNA. A polymerase chain reaction-based sequencing strategy was used to demonstrate that the 16S rRNA sequence can be used for the rapid identification of mycobacterial isolates. Identification at the species level can be obtained within 2 3, requiring <10000 bacteria. This procedure reliably differentiates Mycobacterium spp. which are difficult to identify by classical methods, such as M. Malmoense, M. szulgai and M. flavescens.

引用

页码：1915 / 1920

页数：6

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