ASSOCIATION OF TITIN AND MYOSIN HEAVY-CHAIN IN DEVELOPING SKELETAL-MUSCLE

被引：38

作者：

ISAACS, WB ^{[1
]}

KIM, IS ^{[1
]}

STRUVE, A ^{[1
]}

FULTON, AB ^{[1
]}

机构：

[1] UNIV IOWA,DEPT BIOCHEM,IOWA CITY,IA 52242

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 16期

关键词：

MYOGENESIS; CYTOSKELETON; ASSEMBLY INVIVO;

D O I：

10.1073/pnas.89.16.7496

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To understand molecular interactions that organize developing myofibrils, we examined the biosynthesis and interaction of titin and myosin heavy chain in cultures of developing muscle. Use of pulse-labeling, immunoprecipitation, and a reversible cross-linking procedure demonstrates that within minutes of synthesis, titin and myosin heavy chain can be chemically cross-linked into very large, detergent-resistant complexes retaining many features of intact myotubes. These complexes, predominantly of titin and myosin, occur very early in myofibrillogenesis as well as later. These data suggest that synthesis and assembly of titin and myosin are temporally and spatially coordinated in nascent myofibrils and support the hypothesis that titin molecules help to organize sarcomere formation.

引用

页码：7496 / 7500

页数：5

共 15 条

[1] THE RELATIONSHIP BETWEEN STRESS FIBER-LIKE STRUCTURES AND NASCENT MYOFIBRILS IN CULTURED CARDIAC MYOCYTES [J].